Accurate evaluation of the preservation state of fossil phytoliths in glacial lake sediments is important, as these microfossils are often used in paleoecological and archaeological studies. The characteristic phytolith type of the Norway spruce (Picea abies [L.] Karst.) needle is a potential keystone in paleoecological studies. In this laboratory study, we investigated dissolution of Picea abies blocky type phytoliths, to simulate dissolution processes in sediments and soils and create reference material to compare with fossil phytoliths. Intact needles, needle ash, diatomite and silica gel were treated with Britton–Robinson buffer solutions at pH values from 2 to 12 for 22 days. Silicon was measured by microwave plasma atomic emission spectrometry. Treatment effects were evaluated on longitudinal cuts of needles under a stereomicroscope and on phytolith assemblages from needles using a light microscope. Surfaces of treated phytoliths were investigated by scanning electron microscope and elemental analysis of phytoliths was determined by energy dispersive X-ray fluorescence. Dissolution of silicon in spruce needles was inhibited between pH 8.0 and 11.1. Needle tissue protects phytoliths from erosion processes at this alkaline pH range. Most dissolved silicon appeared to originate from the phytolith surfaces and the silica matrix of the apoplast in the tissues, with less from complete dissolution of phytoliths. Our experiment suggests that extraneous metal elements are incorporated into the silica structure during the dissolution process. Thus, higher element content is an effect of partial dissolution rather than a cause of dissolution. Ultrastructure of the surface of Picea-blocky type phytoliths, namely disappearance of the globular structure, may be useful to assess the intensity of destructive processes in sediments. Our experimental treatments indicate that characteristic Picea-blocky phytoliths in needles can be well-preserved, depending on circumstances in sediments. Further micro-analytical measurements will make these needles promising tools for paleoenvironmental reconstructions.